22. August 2012
Downscaling in Human Cell Culture
In today's highly competitive biopharmaceutical market a shorter time-to-market is crucial for a successful business. Optimization of workflows starts in the early process development and involves cell line screening, clone selection as well as optimization of media, feed components and stategies, and other process parameters. Bioreactors used in this phase should mimic the characteristics of production-scale bioreactors thus allowing for process development in accordance to Quality by Design standards.
In the August issue of the Laboratory Journal Europe, scale-down activities of scientists at CEVEC Pharmaceuticals (Cologne, Germany) are discussed. Using the DASGIP Mini Bioreactor System DASbox equipped with the new DASbox single-use vessel they successfully transferred their existing human cell cultivation process from 500 mL to 170 mL working volume gaining reliable and reproducible results.