Frequently Asked Questions (FAQ)
All users of bioreactor systems have questions, sooner or later. This is due not only to the technical aspects of the product, but also because of the many different ways to analyze and influence biological processes.
The FAQs listed here represent a selection of the questions that we receive most often. We look forward to even more. It's these questions that help us better understand the people who use our products and what their needs are. They also tell us that you use and have an interest in our products. Should you have a question that is not answered to your satisfaction here, don't hesitate to get in touch with us!
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Questions about DASGIP Technology
- What are typical applications for DASGIP Bioreactor Systems?
- How many bioreactors are operated simultaneously using DASGIP?
- How much space does a 4-fold bioreactor system require?
- How many parameters can I control using DASGIP System?
- How many parameters can be displayed in a process overview?
- Do I need programming skills to automate my process?
- Can I change triggers, profiles, cascades and scripts online?
- How difficult is it to modify a DASGIP System subsequently?
- How many speed controlled pumps do the DASGIP Reactors feature?
- Which temperature range can be controlled in a DASGIP Bioreactor?
- How many mass flow sensors are available?
- What is the benefit of the off-gas-analysis?
- How many ports do DASGIP reactors feature?
- Which working volumes are available using DASGIP Control System?
- How is the reactor protected against overheating?
- How are DASGIP vessels agitated?
- Can I integrate DASGIP with Historians & Supervisory Control?
- How can I harness data generated by Parallel Bioreactor System?
- Can I get remote access to a running process outside my lab?
- Are there any alarms in case of critical deviation?
- Why do I need calibration data sheets for the DASGIP BioLector?
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Technical Questions
- I can not log-in into DASGIP Control: what is the reason?
- Can you help me with my controller loop parameters?
- What can I do against the pH drift during longer experiments?
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How to calibrate a pH probe?
According to DIN 19 263 an offset current of 0 ± 30mV at pH7.0 is permissible. Depending on manufacturers and types of electrodes typical values may vary. If the slope value exceeds the range between 95% and 105% the probe should be cleaned.
DASGIP Control software allows for the parallel calibration of all probes used within one system with all data logged in the central database together with set-points, profiles, cascades, process values, user interactions and other events.
The solution used to clean a pH electrode depends on the possible contaminants. Use the guide below to choose the appropriate solution:
- after longer storage in water or air: several hours in 3.0 mol/L Potassium chloride solution
- inorganic deposits: up to 30 minutes in 0.1 mol/L Hydrochloride acid or 0.1 mol/L Sodium hydroxide solution
- oily or organic liquids: rinse with acetone or ethanol
- protein deposits: up to one hour in 1% Pepsin / 0.1M HCl solution
sulphide soiling (black diaphragm): Thiourea / HCl solution (7,5% in 0.1 mol/L) until decolourisation of the diaphragm.
If after cleaning the calibration parameter do not improve a replacement of the probe should be taken in consideration. PH probes are wearing parts. Good maintenance will prolong the lifetime whereas high temperatures (ie during autoclavation), alkaline solutions (ie during alkaline deactivation), repeated etchings and improper maintenance will reduce the electrode's lifetime.
For more information read FAQ technical question on How strong is the influence of temperature for my pH calibration, watch this movie on “How to calibrate pH probes” or contact service@dasgip.de or call +49-700-DASGIP-AG/+49-700-327447-24 - How strong is the influence of temperature for my pH calibration?
- What is a cascade in dissolved oxygen (DO) control?
- What is 100%DO and what is the difference to 100V% oxygen?
- What calibration parameters do you recommend for a DO probe?
- Is it possible to receive a negative OTR in off-gas measurement?
- What are typical process variables to be controlled by triggers?
- How reliable are off-gas data when working at benchtop scale?
- Is it possible to feed continuously even at very low feed rates?
- How to clean Polytetrafluorethylen (PTFE) components?
- How to clean metal sparger?
- How to clean glas sparger?
- How to clean stainless steel components?
- Do stainless steel components bear any risk of corrosion?
- Is it possible to automatically control the foam in DASGIP vessels?
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General Questions
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Glossary